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THETM c-Myc Antibody [HRP], mAb, Mouse

Catalog No. Size Price Figures
A00863-40 ug
 		40 ug
$ 74.00
MSDS:  20081117213314  (PDF)
MSDS:  20090114012119  (PDF)

EXAMPLE:
Mouse Anti-c-Myc monoclonal antibody[HRP]
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A00863-200 ug
 		200 ug
$ 333.00
Full Name
THETM c-Myc Antibody [HRP], mAb, Mouse
AliasMouse Anti-c-Myc [HRP] mAb
DescriptionGenScript THETM c-Myc Antibody [HRP], mAb, Mouse is immunoaffinity purified from mice ascites with protein A and then conjugated to horseradish peroxidase. Mouse Anti-c-Myc [HRP] Monoclonal Antibody is suitable for detecting the expression level of c-Myc in fusion proteins.
Ig SubclassMouse IgG2a
StorageThe antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
SpecificityGenScript THETM c-Myc Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc-tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes the c-Myc gene in human cancers, such as Hela whole cell lysate.
Concentration0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Clone2G8D5
ImmunogenA synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH
Purification MethodProtein A affinity column
ReconstitutionReconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
ApplicationsWorking concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05–0.2 µg/ml
Western blot: 0.1-1 µg/ml
Immunoprecipitation (IP): 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Other applications: user-optimized
Fusion PartnerSpleen cells are fused with SP2/0-Ag14 mouse myeloma cells.
ConjugationHorseradish peroxidase (HRP)
Host SpeciesMouse
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical ManualTM0589, TM0590, TM0591, TM0592, TM0593
* For Non-Clinical Research Use Only *
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